p.UTA.2.0 miR 19b-3p
(Plasmid #82442)

• Purpose: Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p
• Depositing Lab: Jens Gruber
• Publication: Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.

Backbone

• Vector backbone: psiCHECK™-2 Vector
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3660
• Modifications to backbone: Luciferase genes were replaced with CFP and YFP. YFP after the Tk Promoter and CFP after te SV40 promoter. Other regions were changes to full fill correct ORF for the fluorescent proteins and full fill cloning requirements.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hsa-miR-19-3p
• Species: H. sapiens (human)
• Insert Size (bp): 22
• Mutation: Complementary region for hsa-miR-19-3p as sensor
• GenBank ID: NR_029490.1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EYFP from pLenti-CaMKIIa-eArchT 3.0-EYFP (Plasmid #35513) ECFP from pcDNA3-CFP (Plasmid #13030)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the following published protocol for details on plasmid use and data analysis: https://bio-protocol.org/e3000