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p.UTA.2.0-GL-2
(Plasmid #82440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK™-2 Vector
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3660
  • Modifications to backbone
    Luciferase genes were replaced with CFP and YFP. YFP after the Tk Promoter and CFP after te SV40 promoter. Other regions were changes to full fill correct ORF for the fluorescent proteins and full fill cloning requirements.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Insert Size (bp)
    77
  • Mutation
    The complementary region for GL2 siRNA control was inserted three times at the 3 UTR

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EYFP from pLenti-CaMKIIa-eArchT 3.0-EYFP (Plasmid #35513) ECFP from pcDNA3-CFP (Plasmid #13030)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the following published protocol for details on plasmid use and data analysis: https://bio-protocol.org/e3000

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p.UTA.2.0-GL-2 was a gift from Jens Gruber (Addgene plasmid # 82440 ; http://n2t.net/addgene:82440 ; RRID:Addgene_82440)

  • For your References section:

    Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M, Gruber J. Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197. 10.1038/srep45197 PubMed 28338079
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