pEGFP-N1-mt-ro1GFP
(Plasmid
#82407)
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PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 82407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerBD Biosciences Clontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namero1GFP
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Speciesjellyfish
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MutationC48S/F64L/T65S/S147C/Q204C
- Promoter CMV
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Tag
/ Fusion Protein
- leader sequence of the E1α subunit of pyruvate dehydrogenase for mitochondrial targeting (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-mt-ro1GFP was a gift from S. James Remington (Addgene plasmid # 82407 ; http://n2t.net/addgene:82407 ; RRID:Addgene_82407) -
For your References section:
Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, Remington SJ. J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13. 10.1074/jbc.M312846200 PubMed 14722062
