pEGFP-N1-ro2GFP
(Plasmid #82370)

• Purpose: Expression of redox sensitive GFP in mammalian cells
• Depositing Lab: S. James Remington
• Publication: Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: BD Biosciences Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ro2GFP
• Species: jellyfish
• Mutation: C48S/F64L/S147C/Q204C
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: roGFP mutations were introduced into the vector pEGFP-N1 from BD Biosciences Clontech, already encoding an optimized GFP for expression in mammalian cells
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry