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Addgene

pUC19_BAS1509
(Plasmid #82354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 3281
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BAS1509 hypothetical protein
  • Alt name
    BAS1509
  • Species
    Bacillus anthracis str. Sterne
  • Insert Size (bp)
    625
  • GenBank ID
    YP_027776.1 NC_005945.1 (1542527..1543012)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer tttcccagtcacgacgtt
  • 3′ sequencing primer tgtggaattgtgagcgga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_BAS1509 was a gift from Nils Walter (Addgene plasmid # 82354 ; http://n2t.net/addgene:82354 ; RRID:Addgene_82354)

  • For your References section:

    A translational riboswitch coordinates nascent transcription-translation coupling. Chatterjee S, Chauvier A, Dandpat SS, Artsimovitch I, Walter NG. Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). pii: 2023426118. doi: 10.1073/pnas.2023426118. 10.1073/pnas.2023426118 PubMed 33850018
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