FLAG-SLC30A10-E25A
(Plasmid #82345)

• Purpose: Expresses FLAG-tagged SLC30A10-E25A in mammalian cells
• Depositing Lab: Somshuvra Mukhopadhyay
• Publication: Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 10.1074/jbc.M116.726935. Epub 2016 Jun 15.

Backbone

• Vector backbone: pCMV-FLAG10
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 8000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC30A10
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: E25A
• Entrez Gene: SLC30A10 (a.k.a. HMDPC, HMNDYT1, ZNT10, ZNT8, ZRC1, ZnT-10)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: Internal SLC30A10

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SLC30A10-WT originally obtained from Dr. Ruth Valentine. E25A mutation introduced using quick-change.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See for reference to SLC30A10-WT:
SLC30A10 is a cell surface-localized manganese efflux transporter, and parkinsonism-causing mutations block its intracellular trafficking and efflux activity. Leyva-Illades D, Chen P, Zogzas CE, Hutchens S, Mercado JM, Swaim CD, Morrisett RA, Bowman AB, Aschner M, Mukhopadhyay S. J Neurosci. 2014 Oct 15;34(42):14079-95. doi: 10.1523/JNEUROSCI.2329-14.2014. PMID: 25319704