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pLenti-V5-KDM3A-mut
(Plasmid #82332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KDM3A
  • Species
    H. sapiens (human)
  • Mutation
    demethylase-inactive H1120G/D1122N and two silent mutations to make it siRNA resistant
  • Entrez Gene
    KDM3A (a.k.a. JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-V5-KDM3A-mut was a gift from Luke Gaughan (Addgene plasmid # 82332 ; http://n2t.net/addgene:82332 ; RRID:Addgene_82332)

  • For your References section:

    The histone demethylase enzyme KDM3A is a key estrogen receptor regulator in breast cancer. Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan L. Nucleic Acids Res. 2015 Jan;43(1):196-207. doi: 10.1093/nar/gku1298. Epub 2014 Dec 8. 10.1093/nar/gku1298 PubMed 25488809
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