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pDONR223_NRAS_WT
(Plasmid #82151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Gateway Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRAS
  • Alt name
    NM_002524.4
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Closed state: stop codon immediately follows insert Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223_NRAS_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82151 ; http://n2t.net/addgene:82151 ; RRID:Addgene_82151)

  • For your References section:

    Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. 10.1158/2159-8290.CD-16-0160 PubMed 27147599
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