pICE-HA-MRE11-H63D
(Plasmid #82036)

• Purpose: Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
• Depositing Labs: Sébastien Britton, Patrick Calsou
• Publication: Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.

Backbone

• Vector backbone: pICE
• Backbone manufacturer: Synthetic - Addgene Plasmid #46960
• Backbone size w/o insert (bp): 5023
• Total vector size (bp): 7150
• Modifications to backbone: HA Tag and cloning sites
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MRE11
• Alt name: MRE11A
• Species: H. sapiens (human)
• Insert Size (bp): 2127
• Mutation: Exonuclease-dead mutant: H63D MRE11
• Entrez Gene: MRE11 (a.k.a. ATLD, HNGS1, MRE11A, MRE11B)
• Promoter: CMV-tet
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Resource Information

• Supplemental Documents:
  • Annotated GenBank sequence
• A portion of this plasmid was derived from a plasmid made by: I.M.A.G.E. clone #5181691
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Important note: this mutant was originally incorrectly described as MRE11-H63N in the associated publication. A corrigendum is upcoming.