pICE-HA-CtIP-siR-S664A-S679A-S745A
(Plasmid #82031)

• Purpose: Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
• Depositing Labs: Sébastien Britton, Patrick Calsou
• Publication: Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.

Backbone

• Vector backbone: pICE
• Backbone manufacturer: Synthetic - Addgene plasmid #46960
• Backbone size w/o insert (bp): 5011
• Total vector size (bp): 7705
• Modifications to backbone: HA Tag and cloning sites
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CtIP
• Alt name: RBBP8
• Alt name: hSae2
• Species: H. sapiens (human)
• Insert Size (bp): 2752
• Mutation: Non phosphorylatable mutations on 3 of the main ATM-dependent phosphorylation sites: S664A+S679A+S745A; Mutations making the cDNA resistant to a siRNA
• Entrez Gene: RBBP8 (a.k.a. COM1, CTIP, JAWAD, JWDS, RIM, SAE2, SCKL2)
• Promoter: CMV-tet
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Resource Information

• Supplemental Documents:
  • Author annotated GenBank sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry