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Addgene

pACSA_Pcpt_YFP
(Plasmid #82019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACSA
  • Total vector size (bp) 5008

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YFP
  • Promoter Pcpt

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCTTCCGGCTCGTATGTTGTG
  • 3′ sequencing primer TTGGGTAACGCCAGGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACSA_Pcpt_YFP was a gift from Brian Pfleger (Addgene plasmid # 82019 ; http://n2t.net/addgene:82019 ; RRID:Addgene_82019)

  • For your References section:

    Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF. ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25. 10.1021/sb500260k PubMed 25216157
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