pDONR223_KRAS_p.G12V
(Plasmid #81665)

• Purpose: Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.
• Depositing Labs: Jesse Boehm, William Hahn, David Root
• Publication: Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2790
• Vector type: Gateway Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KRAS
• Alt name: NM_004985.4
• Species: H. sapiens (human)
• Mutation: G12V
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Promoter: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Closed state: stop codon immediately follows insert