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Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA
(Plasmid #81254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lentiviral
  • Total vector size (bp) 10512
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    stgRNA
  • gRNA/shRNA sequence
    40nt sgRNA
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA was a gift from Timothy Lu (Addgene plasmid # 81254 ; http://n2t.net/addgene:81254 ; RRID:Addgene_81254)

  • For your References section:

    Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Perli SD, Cui CH, Lu TK. Science. 2016 Aug 18. pii: aag0511. 10.1126/science.aag0511 PubMed 27540006
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