Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR
(Plasmid
#81253)
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PurposeLentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentiviral
- Total vector size (bp) 11427
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTetR and LacI
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR was a gift from Timothy Lu (Addgene plasmid # 81253 ; http://n2t.net/addgene:81253 ; RRID:Addgene_81253) -
For your References section:
Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Perli SD, Cui CH, Lu TK. Science. 2016 Aug 18. pii: aag0511. 10.1126/science.aag0511 PubMed 27540006