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Addgene

Construct 1 - U6p_20nt1_WT_sgRNA
(Plasmid #81245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lentiviral
  • Total vector size (bp) 10777
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • gRNA/shRNA sequence
    20nt sgRNA
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Construct 1 - U6p_20nt1_WT_sgRNA was a gift from Timothy Lu (Addgene plasmid # 81245 ; http://n2t.net/addgene:81245 ; RRID:Addgene_81245)

  • For your References section:

    Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Perli SD, Cui CH, Lu TK. Science. 2016 Aug 18. pii: aag0511. 10.1126/science.aag0511 PubMed 27540006
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