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PurposeA ZipGFP-based Caspase-3 protease reporter T2A mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZipGFP with caspase cleavage sequence T2A mCherry
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SpeciesSynthetic
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZipGFP-Casp3 was a gift from Xiaokun Shu (Addgene plasmid # 81241 ; http://n2t.net/addgene:81241 ; RRID:Addgene_81241) -
For your References section:
Rational Design of a GFP-Based Fluorogenic Caspase Reporter for Imaging Apoptosis In Vivo. To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin SR, Shu X. Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 10.1016/j.chembiol.2016.06.007. 10.1016/j.chembiol.2016.06.007 PubMed 27447051