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p5E-mitfa2.1
(Plasmid #81234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p5E-MCS from Tol2kit, derived from pDONR P4-P1R
  • Backbone manufacturer
    Invitrogen
  • Vector type
    multisite Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitfa promoter
  • Alt name
    3A.1
  • Alt name
    nacre
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2126
  • GenBank ID
    30080
  • Entrez Gene
    mitfa (a.k.a. nacre, z3A.1)
  • Promoter mitfa, 2.1kb including 5' UTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (destroyed during cloning)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

p5E-MCS, from which this plasmid was constructed, is from the Tol2kit, described in Kwan et al. (2007) Developmental Dynamics 236:3088-3099

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-mitfa2.1 was a gift from James Lister (Addgene plasmid # 81234 ; http://n2t.net/addgene:81234 ; RRID:Addgene_81234)
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