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pCS2-MT-mitfa
(Plasmid #81231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2-MT
  • Vector type
    Mammalian Expression ; zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitfa
  • Species
    D. rerio (zebrafish)
  • Mutation
    contains amino acids 5-412
  • Entrez Gene
    mitfa (a.k.a. nacre, z3A.1)
  • Promoter sCMV IE94 promoter
  • Tag / Fusion Protein
    • 6 myc tags (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also called pCS2-MT3A.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-MT-mitfa was a gift from James Lister & David Raible (Addgene plasmid # 81231 ; http://n2t.net/addgene:81231 ; RRID:Addgene_81231)

  • For your References section:

    nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Lister JA, Robertson CP, Lepage T, Johnson SL, Raible DW. Development. 1999 Sep;126(17):3757-67. PubMed 10433906
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