pBS-mitfa
(Plasmid
#81230)
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PurposeCloning of zebrafish mitfa gene
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK(-)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitfa
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SpeciesD. rerio (zebrafish)
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Entrez Genemitfa (a.k.a. nacre, z3A.1)
- Promoter T3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-mitfa was a gift from James Lister & David Raible (Addgene plasmid # 81230 ; http://n2t.net/addgene:81230 ; RRID:Addgene_81230) -
For your References section:
nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Lister JA, Robertson CP, Lepage T, Johnson SL, Raible DW. Development. 1999 Sep;126(17):3757-67. PubMed 10433906