pNP-P+
(Plasmid
#81229)
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Purpose(Empty Backbone) the CMV promoter of pCS2 has been replaced by a 931-bp fragment of the mitfa promoter and 5'UTR
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 81229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
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Modifications to backboneCMV promoter of pCS2 has been replaced by a 931-bp fragment of the mitfa promoter and 59UTR via the SalI and HindIII sites
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Vector typeMammalian Expression ; xenopus//avian/zebrafish
- Promoter mitfa
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also called pNP
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP-P+ was a gift from James Lister & David Raible (Addgene plasmid # 81229 ; http://n2t.net/addgene:81229 ; RRID:Addgene_81229) -
For your References section:
Duplicate mitf genes in zebrafish: complementary expression and conservation of melanogenic potential. Lister JA, Close J, Raible DW. Dev Biol. 2001 Sep 15;237(2):333-44. 10.1006/dbio.2001.0379 PubMed 11543618
