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Addgene

pTIM
(Plasmid #81198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIDTSmart Amp
  • Backbone manufacturer
    Integrated DNA Technologies
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Integrated DNA Technologies

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the plasmid maps (second and third thumbnails below the large map image) for detailed sequence annotations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTIM was a gift from Andrés Vázquez-Torres (Addgene plasmid # 81198 ; http://n2t.net/addgene:81198 ; RRID:Addgene_81198)

  • For your References section:

    Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region. Tapscott T, Kim JS, Crawford MA, Fitzsimmons L, Liu L, Jones-Carson J, Vazquez-Torres A. Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.1038/s41598-018-27780-9. 10.1038/s41598-018-27780-9 PubMed 29930310
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