D. vulgaris sp2 CRISPR/pACYCDuet-1
(Plasmid #81186)

• Purpose: Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli
• Depositing Lab: Jennifer Doudna
• Publication: Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi: 10.1016/j.molcel.2016.07.027.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4008
• Total vector size (bp): 3896
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
• gRNA/shRNA sequence: TTGATGAGTGGCGCACTCGCCAGCCTGAGCATGGC
• Species: Desulfovibrio vulgaris str. Hildenborough
• Insert Size (bp): 873
• GenBank ID:
• Promoter: T7
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoNI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: Custom
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Spacer sequence: GCCATGCTCAGGCTGGCGAGTGCGCCACTCATCAA
Repeat sequence: GTCGCCCCCCACGCGGGGGCGTGGATTGAAAC