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D. vulgaris Cas5c/pHMGWA
(Plasmid #81182)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHMGWA
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 8236
  • Total vector size (bp) 7331
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas5c
  • Alt name
    Cas5d
  • Alt name
    Cas5
  • Species
    Desulfovibrio vulgaris str. Hildenborough
  • Insert Size (bp)
    680
  • GenBank ID
    2781521 YP_009170
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis - MBP - TEV protease site (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D. vulgaris Cas5c/pHMGWA was a gift from Jennifer Doudna (Addgene plasmid # 81182 ; http://n2t.net/addgene:81182 ; RRID:Addgene_81182)

  • For your References section:

    DNA Targeting by a Minimal CRISPR RNA-Guided Cascade. Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA. Mol Cell. 2016 Sep 1;63(5):840-51. doi: 10.1016/j.molcel.2016.07.027. 10.1016/j.molcel.2016.07.027 PubMed 27588603
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