pRetro-XT-PAK2-CFP(puro)-T402A
(Plasmid #81179)

• Purpose: Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion
• Depositing Lab: Angeliki Malliri
• Publication: Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.1038/ncomms8437.

Backbone

• Vector backbone: pRetroX-Tight-Pur
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6567
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p21 (RAC1) activated kinase 2
• Alt name: PAK2
• Species: H. sapiens (human)
• Mutation: T402A
• Entrez Gene: PAK2 (a.k.a. KNO2, PAK65, PAKgamma)
• Promoter: P-tight TRE
• Tag / Fusion Protein:
  • CFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: NruI (unknown if destroyed)
• 5′ sequencing primer: pBABE 5
• 3′ sequencing primer: MSCV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry