MCV
(Plasmid
#81174)
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Purpose(Empty Backbone) MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCV
- Backbone size (bp) 1925
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCV was a gift from Serge Pelet (Addgene plasmid # 81174 ; http://n2t.net/addgene:81174 ; RRID:Addgene_81174) -
For your References section:
New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast. Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S. Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. 10.1007/s00438-016-1249-1 [pii] PubMed 27637489