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Purposeidentification of cell-specific targets of RNA binding proteins
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJFRC7-20XUAS-IVS-mCD8::GFP
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUAS-ADARcd
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SpeciesD. melanogaster (fly)
- Promoter UAS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40-splice-R2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJFRC-20X-UAS-ADARcd was a gift from Michael Rosbash (Addgene plasmid # 81173 ; http://n2t.net/addgene:81173 ; RRID:Addgene_81173) -
For your References section:
TRIBE: Hijacking an RNA-Editing Enzyme to Identify Cell-Specific Targets of RNA-Binding Proteins. McMahon AC, Rahman R, Jin H, Shen JL, Fieldsend A, Luo W, Rosbash M. Cell. 2016 Mar 30. pii: S0092-8674(16)30263-X. doi: 10.1016/j.cell.2016.03.007. 10.1016/j.cell.2016.03.007 PubMed 27040499