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pMT-Hrp48-ADARcd-V5
(Plasmid #81172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-V5-His A B C
  • Vector type
    Insect Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hrp48-ADARcd fusion
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Hrb27C (a.k.a. Dmel_CG10377, 10280, CG10377, Dmel\CG10377, HRB27C, HRP48, Hrb27-C, Hrb27C/Hrp48, Hrb27c, Hrb48, Hrp48, Hrp48/Hrb27C, Ov5, RRM7, Rbp7, hnRNP27C, hnRNP48, hrb27, hrb27C, hrb27c, hrp48, l(2)02647, l(2)10280, l(2)2647, linha, p50, rrm7)
  • Promoter Drosophila metallothionein promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer MT-Forward
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Hrp48-ADARcd-V5 was a gift from Michael Rosbash (Addgene plasmid # 81172 ; http://n2t.net/addgene:81172 ; RRID:Addgene_81172)

  • For your References section:

    TRIBE: Hijacking an RNA-Editing Enzyme to Identify Cell-Specific Targets of RNA-Binding Proteins. McMahon AC, Rahman R, Jin H, Shen JL, Fieldsend A, Luo W, Rosbash M. Cell. 2016 Mar 30. pii: S0092-8674(16)30263-X. doi: 10.1016/j.cell.2016.03.007. 10.1016/j.cell.2016.03.007 PubMed 27040499
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