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pET29NK-LGK/mGFPmut3
(Plasmid #81168)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81168 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29NK
  • Backbone manufacturer
    pET29b Derivative
  • Backbone size w/o insert (bp) 5961
  • Total vector size (bp) 7278
  • Modifications to backbone
    After the XhoI site, mGFPmut3 is cloned in-frame. After mGFPmut3 is the His6x tag, stop codon and then the BbvCI nicking site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Levoglucosan kinase wild-type
  • Alt name
    B3VI55_LIPST
  • Species
    Synthetic; Lipomyces starkeyi
  • Insert Size (bp)
    1317
  • GenBank ID
    ACE79748.1 EU751287.1
  • Promoter T7
  • Tags / Fusion Proteins
    • mGFPmut3 (C terminal on backbone)
    • His6x (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29NK-LGK/mGFPmut3 was a gift from Timothy Whitehead (Addgene plasmid # 81168 ; http://n2t.net/addgene:81168 ; RRID:Addgene_81168)

  • For your References section:

    An Automated Data-Driven Pipeline for Improving Heterologous Enzyme Expression. Wrenbeck EE, Bedewitz MA, Klesmith JR, Noshin S, Barry CS, Whitehead TA. ACS Synth Biol. 2019 Mar 15;8(3):474-481. doi: 10.1021/acssynbio.8b00486. Epub 2019 Feb 8. 10.1021/acssynbio.8b00486 PubMed 30721031
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