pTriEx-mVenus-Zdk1-Rac1 Q61L
(Plasmid #81137)

• Purpose: mammalian expression of Zdk1 tagged Rac1 Q61L
• Depositing Lab: Klaus Hahn
• Publication: Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6000
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Zdk1
• Alt name: ZA127
• Species: Synthetic
• Insert Size (bp): 177
• Tag / Fusion Protein:
  • mVenus (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Gene/Insert 2

• Gene/Insert name: Rac1
• Species: H. sapiens (human)
• Insert Size (bp): 576
• Mutation: Q61L
• GenBank ID: AF498964.1
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry