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pGL2-Skp2 promoter-luciferase
(Plasmid #81119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SKP2
  • Alt name
    FBXL1
  • Species
    M. musculus (mouse)
  • Mutation
    contains the region from 1_kb upstream of transcription start site to 200_bp downstream
  • Entrez Gene
    Skp2 (a.k.a. 4930500A04Rik, FBXL1, FWD1, p45)
  • Promoter SKP2
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer F1ori-F
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-Skp2 promoter-luciferase was a gift from Sung Hee Baek (Addgene plasmid # 81119 ; http://n2t.net/addgene:81119 ; RRID:Addgene_81119)

  • For your References section:

    AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Shin HR, Kim H, Oh S, Lee JG, Kee M, Ko HJ, Kweon MN, Won KJ, Baek SH. Nature. 2016 Jun 15. doi: 10.1038/nature18014. 10.1038/nature18014 PubMed 27309807
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