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Addgene

pcDNA-1xHA CARM1 WT
(Plasmid #81118)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARM1
  • Alt name
    PRMT4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Carm1 (a.k.a. Prmt4, m9Bei)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer bGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T480A substitution found during QC does not affect the function of the
protein CARM1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-1xHA CARM1 WT was a gift from Sung Hee Baek (Addgene plasmid # 81118 ; http://n2t.net/addgene:81118 ; RRID:Addgene_81118)

  • For your References section:

    AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Shin HR, Kim H, Oh S, Lee JG, Kee M, Ko HJ, Kweon MN, Won KJ, Baek SH. Nature. 2016 Jun 15. doi: 10.1038/nature18014. 10.1038/nature18014 PubMed 27309807
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