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Purposemammalian expression of Skp2 WT
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 81115 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3xFlag-CMV10
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Backbone manufacturerSIGMA-ALDRICH
- Backbone size w/o insert (bp) 6299
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSKP2
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Alt nameFBXL1; SKP2 isoform 2
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SpeciesH. sapiens (human)
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GenBank IDNM_032637.3
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Entrez GeneSKP2 (a.k.a. FBL1, FBXL1, FLB1, p45)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV10-3xFlag Skp2 WT was a gift from Sung Hee Baek (Addgene plasmid # 81115 ; http://n2t.net/addgene:81115 ; RRID:Addgene_81115) -
For your References section:
AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Shin HR, Kim H, Oh S, Lee JG, Kee M, Ko HJ, Kweon MN, Won KJ, Baek SH. Nature. 2016 Jun 15. doi: 10.1038/nature18014. 10.1038/nature18014 PubMed 27309807
