GTH-y
(Plasmid #81105)

• Purpose: Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
• Depositing Lab: Serge Pelet
• Publication: Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.

Backbone

• Vector backbone: pSIVh
• Total vector size (bp): 4305
• Vector type: Yeast Expression, Cre/Lox
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCitrine
• Alt name: mCitrine (A206K L221K)
• Insert Size (bp): 760
• Mutation: Mutated mCitrine for monomeric isoform (A206K L221K)
• Tag / Fusion Protein:
  • mCitrine (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry