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Addgene

GTH-g
(Plasmid #81104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIVh
  • Total vector size (bp) 4296
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Alt name
    superfolder GFP
  • Insert Size (bp)
    750
  • Tag / Fusion Protein
    • sfGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GTH-g was a gift from Serge Pelet (Addgene plasmid # 81104 ; http://n2t.net/addgene:81104 ; RRID:Addgene_81104)

  • For your References section:

    New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast. Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S. Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. 10.1007/s00438-016-1249-1 [pii] PubMed 27637489
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