SIVl
(Plasmid
#81090)
-
Purpose(Empty Backbone) Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 81090 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneSIVl
- Backbone size (bp) 4391
-
Vector typeYeast Expression, Cre/Lox
-
Selectable markersLEU2
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SIVl was a gift from Serge Pelet (Addgene plasmid # 81090 ; http://n2t.net/addgene:81090 ; RRID:Addgene_81090) -
For your References section:
New families of single integration vectors and gene tagging plasmids for genetic manipulations in budding yeast. Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S. Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16. 10.1007/s00438-016-1249-1 [pii] PubMed 27637489