rAAV2-retro helper
(Plasmid #81070)

• Purpose: retrograde AAV helper vector, carrying rep and modified capsid gene
• Depositing Labs: Alla Karpova, David Schaffer
• Publication: Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 10.1016/j.neuron.2016.09.021.

Backbone

• Vector backbone: AAV helper vector
• Vector type: AAV, Unspecified ; helper vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 or Stbl2 are recommended by depositing lab. This plasmid may be prone to recombination. Please process the stab immediately by streaking on a bacterial plate and testing individual colonies for accuracy. Prepare DNA from a freshly plated colony.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: rep
• Species: adeno-associated virus
• Insert Size (bp): 1865
• Mutation: Pro2Ala, possibly Asp613Gly

Gene/Insert 2

• Gene/Insert name: rAAV2-retro cap
• Species: adeno-associated virus
• Insert Size (bp): 2237
• Mutation: changes made to AAV2 cap: N382D, V708I, insertion of LADQDYTKTA between N587 and R588

Resource Information

• Supplemental Documents:
  • Genbank
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 38 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The uncut plasmid runs as a multimer (>10kb). Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If the monomer is needed, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

this vector was generated as a part of the Janelia viral project team in collaboration with David V. Schaffer (Berkeley)

The Janelia Virus Core will be posting their production protocol and general tips for maximizing virus titer on their website: https://www.janelia.org/support-team/virus-services

For a list Addgene viruses packaged with this construct, please visit https://www.addgene.org/viral-service/aav-prep/retrograde/