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pENTR221 UBR5 ∆HECT
(Plasmid #81065)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2522
  • Total vector size (bp) 10958
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT
  • Alt name
    EDD, EDD1, hHYD, DD5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8436
  • Mutation
    C2768A (bp t8302g and g8303c)
  • Entrez Gene
    UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
  • Promoter N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA derived from pEGFP-C1 EDD (already submitted to addgene: https://www.addgene.org/37190/)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR221 UBR5 ∆HECT was a gift from Darren Saunders (Addgene plasmid # 81065 ; http://n2t.net/addgene:81065 ; RRID:Addgene_81065)

  • For your References section:

    The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia. Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, Stokke T, Patzke S, Saunders DN. Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248. 10.1091/mbc.E17-04-0248 PubMed 29742019
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