pENTR221 UBR5 ∆PABC
(Plasmid
#81064)
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PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2522
- Total vector size (bp) 10958
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC
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Alt nameEDD, EDD1, hHYD, DD5
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SpeciesH. sapiens (human)
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Insert Size (bp)8436
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MutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a7243g, a7244c and a7245c)
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Entrez GeneUBR5 (a.k.a. DD5, EDD, EDD1, HYD)
- Promoter N/A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA derived from pEGFP-C1 EDD (already submitted to addgene: https://www.addgene.org/37190/)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221 UBR5 ∆PABC was a gift from Darren Saunders (Addgene plasmid # 81064 ; http://n2t.net/addgene:81064 ; RRID:Addgene_81064) -
For your References section:
The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia. Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, Stokke T, Patzke S, Saunders DN. Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248. 10.1091/mbc.E17-04-0248 PubMed 29742019