pENTR221 UBR5
(Plasmid #81062)

• Purpose: Gateway entry vector encoding full length UBR5
• Depositing Lab: Darren Saunders
• Publication: Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2522
• Total vector size (bp): 10958
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ubiquitin protein ligase E3 component N-recognin 5 (UBR5)
• Alt name: EDD, EDD1, hHYD, DD5
• Species: H. sapiens (human)
• Insert Size (bp): 8436
• Entrez Gene: UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
• Promoter: N/A

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pENTR221 UBR5 snapgene
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: cDNA derived from pEGFP-C1 EDD (already submitted to addgene: https://www.addgene.org/37190/)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry