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Addgene

pTG-mTDGa.1
(Plasmid #81049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTXB3
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6550
  • Total vector size (bp) 7818
  • Modifications to backbone
    Mxe GyrA intein-CBD replaced by PreScission cleavage site and GST
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TDG
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1263
  • Mutation
    asparagine 151 changed to alanine
  • GenBank ID
    NM_011561.2
  • Entrez Gene
    Tdg (a.k.a. E130317C12Rik, JZA-3, Jza, Jza1)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7_promoter
  • 3′ sequencing primer T7_terminal_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTG-mTDGa.1 was a gift from Primo Schaer (Addgene plasmid # 81049 ; http://n2t.net/addgene:81049 ; RRID:Addgene_81049)

  • For your References section:

    Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism. Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, Schuermann D, Klungland A, Schar P. Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806. 10.1038/ncomms10806 PubMed 26932196
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