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Addgene

pHS3
(Plasmid #81038)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 8690
  • Total vector size (bp) 9198
  • Modifications to backbone
    First, 3.8 kb EcoRI-SalI fragment from YCp50-HO containing HO gene (with promoter) cloned into EcoRI-SalI site in pRS316. MCS may not contain unique restriction sites. The plasmid does not have a ApaI site except in URA3. Next, 0.8kb URA3 cassette replaced with 1.3kb NAT cassette.
  • Vector type
    Yeast Expression
  • Selectable markers
    NAT (select with Nourseothricin/clonNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NATMX - HO endonuclease
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1308
  • Promoter TEF

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains HO sequence with its endogenous promoter. Can be used for mating type switch, diploidization.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS3 was a gift from John McCusker (Addgene plasmid # 81038 ; http://n2t.net/addgene:81038 ; RRID:Addgene_81038)
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