pHS2
(Plasmid #81037)

• Purpose: HO endonuclease expression
• Depositing Lab: John McCusker
• Publication: McCusker Lab Unpublished Plasmids (unpublished)

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 8690
• Total vector size (bp): 9654
• Modifications to backbone: First, 3.8 kb EcoRI-SalI fragment from YCp50-HO containing HO gene (with promoter) cloned into EcoRI-SalI site in pRS316. MCS may not contain unique restriction sites. The plasmid does not have a ApaI site except in URA3. Next, 0.8kb URA3 cassette replaced with 1.764kb HYG cassette.
• Vector type: Yeast Expression
• Selectable markers: Hygromycin ; hph( resistance)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HYGMX
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1764
• Promoter: TEF

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid contains HO sequence with its endogenous promoter. Can be used for mating type switch, diploidization.
Please note the Addgene verified sequence differs from the original diagram map.