pTriEx-mCherry-LOV2 I427T
(Plasmid #81035)

• Purpose: Mammalian expression of LOV I427T, a mutant with super short relaxation time
• Depositing Lab: Klaus Hahn
• Publication: Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5040
• Total vector size (bp): 6000
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LOV I427T
• Species: Synthetic
• Insert Size (bp): 432
• Mutation: I427T
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry