SIRT5H158Y-pET15b
(Plasmid #81032)

• Purpose: Bacterial expression of Human catalytic mutant SIRT5 protein
• Depositing Lab: David Lombard
• Publication: Kumar et al Methods Mol Biol. 2016;1436:241-57. doi: 10.1007/978-1-4939-3667-0_16.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 6500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Sirt5
• Species: H. sapiens (human)
• Insert Size (bp): 825
• Mutation: H158Y catalytic mutant
• GenBank ID: 23408
• Entrez Gene: SIRT5 (a.k.a. SIR2L5)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Drs. Marcia Haigis and Natalie German, Harvard Medical School

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert doesn't have mitochondrial targeting sequence.