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Addgene

BAP1 C91A
(Plasmid #81025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-Thy1.1 DEST
  • Backbone manufacturer
    Rao lab (Addgene plasmid# 17442)
  • Backbone size w/o insert (bp) 8200
  • Total vector size (bp) 10400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Thy1.1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    BAP1 C91A (point mutation, catalytically inactive)
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Promoter 5’LTR

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pLXSN-5 (5'-CCCTTGAACCTCCTCGTTCGACC-3')
  • 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAP1 C91A was a gift from Anjana Rao (Addgene plasmid # 81025 ; http://n2t.net/addgene:81025 ; RRID:Addgene_81025)

  • For your References section:

    Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex. Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM, Lahdesmaki H, Rao A. Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307. 10.1038/ncomms8307 PubMed 26095772
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