exon K-renilla
(Plasmid
#81007)
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Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 81007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAc5.1
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Backbone manufacturerInvitrogen
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameparalytic
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Alt namepara
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Alt nameDmNav
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SpeciesD. melanogaster (fly)
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Mutationspanning exon 24 to exon 26, termination codon inserted in exon L
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Entrez Genepara (a.k.a. Dmel_CG9907, CG9907, DmNa[[V]], DmNa[[v]], DmNa[[v]]1, DmNav, DmNav1, Dmel\CG9907, Nav1, Ocd, Para, Scn1alpha, Shu, Shudderer, VSSC, bas, bss, l(1)14Da, l(1)ESHS48, lincRNA.S9469, olfD, par, para13-5, sbl, sbl-1)
- Promoter P-AC5 (Actin 5C)
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Tag
/ Fusion Protein
- Renilla luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer AC5
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
exon K-renilla was a gift from Richard Baines (Addgene plasmid # 81007 ; http://n2t.net/addgene:81007 ; RRID:Addgene_81007) -
For your References section:
Seizure suppression through manipulating splicing of a voltage-gated sodium channel. Lin WH, He M, Baines RA. Brain. 2015 Apr;138(Pt 4):891-901. doi: 10.1093/brain/awv012. Epub 2015 Feb 12. 10.1093/brain/awv012 PubMed 25681415