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Addgene

exon L-firefly
(Plasmid #81006)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81006 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    paralytic
  • Alt name
    para
  • Alt name
    DmNav
  • Species
    D. melanogaster (fly)
  • Mutation
    spanning exon 24 to exon 26, termination codon inserted in exon K
  • Entrez Gene
    para (a.k.a. Dmel_CG9907, CG9907, DmNa[[V]], DmNa[[v]], DmNa[[v]]1, DmNav, DmNav1, Dmel\CG9907, Nav1, Ocd, Para, Scn1alpha, Shu, Shudderer, VSSC, bas, bss, l(1)14Da, l(1)ESHS48, lincRNA.S9469, olfD, par, para13-5, sbl, sbl-1)
  • Promoter P-AC5 (Actin 5C)
  • Tag / Fusion Protein
    • firefly luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AC5
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    exon L-firefly was a gift from Richard Baines (Addgene plasmid # 81006 ; http://n2t.net/addgene:81006 ; RRID:Addgene_81006)

  • For your References section:

    Seizure suppression through manipulating splicing of a voltage-gated sodium channel. Lin WH, He M, Baines RA. Brain. 2015 Apr;138(Pt 4):891-901. doi: 10.1093/brain/awv012. Epub 2015 Feb 12. 10.1093/brain/awv012 PubMed 25681415
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