pIMP1-Pthl-AbrB3647
(Plasmid
#80981)
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PurposeUsed for abrB3647(CAC3647) overexpression in Clostridium acetobutylicum
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIMP1-Pthl
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Backbone manufacturerXiao et al, PMID 21926197
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameabrB3647(CAC3647)
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Insert Size (bp)246
- Promoter thl (cac2873)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIMP1-Pthl-AbrB3647 was a gift from Wei-Hong Jiang (Addgene plasmid # 80981 ; http://n2t.net/addgene:80981 ; RRID:Addgene_80981) -
For your References section:
Roles of three AbrBs in regulating two-phase Clostridium acetobutylicum fermentation. Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y. Appl Microbiol Biotechnol. 2016 Jun 8. 10.1007/s00253-016-7638-x [pii] PubMed 27276910