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Purposemammalian expression of CRT-GFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneGFP-pcDNA3.1
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Backbone manufacturerSteyger lab
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Modifications to backboneGFP with the addition of KDEL was generated by PCR using pEGFP-N2 (Clontech) as a template, digested with NotI/XbaI and subcloned into pcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRT
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Alt namecalreticulin
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SpeciesM. musculus (mouse)
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Mutationno KDEL
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Entrez GeneCalr (a.k.a. CRT, Calregulin)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CRT-GFP pcDNA3.1 was a gift from Peter Steyger (Addgene plasmid # 80978 ; http://n2t.net/addgene:80978 ; RRID:Addgene_80978) -
For your References section:
Calreticulin binds to gentamicin and reduces drug-induced ototoxicity. Karasawa T, Wang Q, David LL, Steyger PS. Toxicol Sci. 2011 Dec;124(2):378-87. doi: 10.1093/toxsci/kfr196. Epub 2011 Jul 23. 10.1093/toxsci/kfr196 PubMed 21785162