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Purposemammalian expression of CLIMP-63
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCLIMP-63
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SpeciesH. sapiens (human)
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Entrez GeneCKAP4 (a.k.a. CLIMP-63, CLIMP63, ERGIC-63, p63)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CLIMP-63 pcDNA was a gift from Peter Steyger (Addgene plasmid # 80977 ; http://n2t.net/addgene:80977 ; RRID:Addgene_80977) -
For your References section:
CLIMP-63 is a gentamicin-binding protein that is involved in drug-induced cytotoxicity. Karasawa T, Wang Q, David LL, Steyger PS. Cell Death Dis. 2010 Nov 18;1:e102. doi: 10.1038/cddis.2010.80. 10.1038/cddis.2010.80 PubMed 21368867
